White Papers

Here is the SPOT Imaging online archive of longer technical articles and white papers on life science imaging subjects.

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Article Abstract
Improve Patient Outcomes with Video Pathology Consultations: A Literature Review A review of published studies indicates that video conferencing support of intraoperative pathology consultation contributes not only to improved patient outcomes, but also reduces liability and results in significant time savings for the hospital, pathology lab and surgical personnel.
How to Obtain Parfocality Between Camera and Microscope Eyepieces Instructions for configuring your microscope imaging system so that the camera image is in focus at the same time as the image viewed through the microscope eyepieces.
How to Set Koehler Illumination Instructions for setting up a microscope for Koehler illumination to get the best image resolution and contrast.
The Effect of the Microscope Adapter Magnification on Image Field of View Technical note on how the microscope adapter magnification effects the resulting image field of view.
How To Calculate The Total Magnification Technical note on how to measure the total magnification of the specimen displayed on your monitor, and how to approximate the real size of your specimen.
Color
Space in Microscope Digital Imaging
An understanding of color space can help you enhance
the quality of your images. In this article we will review three that
are commonly used in digital imaging, RGB, HSL and HSV.
Scientific
Digital Imaging Key Concepts
As fun as taking pictures may be, it is the professional
analysis of samples that really counts. This article discusses key concepts
of imaging systems and how these concepts contribute to a professional
presentation of your work.
The Consumer
Digital Camera vs. Scientific Digital Cameras
We have all seen the recent improvements in consumer
digital cameras. They have gone from low resolution, curiosities to
very capable, high resolution, point and shoot cameras. The prices have
dropped dramatically making them even more enticing. Do these cameras
have a place in pathology?
How To Improve Color Reproduction in Microscope Images When you capture an image of a specimen,
you want it to look like what you saw through the eyepieces.  In
this article we will cover the basic concepts of color management and
what you can do to make sure what you capture is as close to what you
see as possible.
3-Shot vs. Color Mosaic This article illustrates the advantages and disadvantages
of Color Mosaic and 3-Shot sampling technologies
Image Corrections In this discussion we will explore how variations due
to practical hardware considerations are scientifically accounted for
and removed, with the goal of providing an image that is the closest
match to the specimen.
Megapixels Per Dollar Consumer marketing has hyped “mega pixels” as the hook
on which they hang their hat. In scientific imaging, the number of pixels
is only part of the story, in fact, not always the most important part.
This article discusses some of the important specifications of pixels
and their relationship to real world scientific imaging.
Dynamic Range in Digital Imaging How dynamic range affects the aesthetics of images
for documentation, and what effect high dynamic range has on the quantitative
imaging process.