Microscopy Imaging Software
All SPOT cameras come with SPOT Basic software, the user friendly imaging application that makes it simple to capture high quality images.
You can expand your capabilities by adding the modular SPOT Advanced software, which provides tools for fluorescence imaging and database archival, and supports modules for intensity display scaling, extended depth of focus processing, and peripheral device control.
Image analysis tasks like automated counting, deconvolution, thresholding and multi-dimensional imaging can also be accomplished with your SPOT camera using MetaMorph software and the SPOT camera driver.
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SPOT 5.0 Basic SoftwareThe SPOT Basic software is a comprehensive but straighforward interface with features designed for microscopy imaging. Its interface includes preconfigured settings for different microscope illumination types so that the camera is optimized for your specific use. Image previewing is enhanced with the magnifier and focus gauge in the SPOT Basic live window interface. It provides user-friendly reports, reticles, measurements, calibration marks, time lapse, annotations, and automatic white balance features that make it simple to acquire impressive images. |
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SPOT 5.0 Advanced SoftwareThe SPOT Advanced microscopy software expands your imaging toolkit with a customizable database for archival, merging and pseudo-coloring of monochrome fluorescence channels, custom dialog boxes, variables and taskbars, macro scripting, live histogram, focus drift viewer for time lapse acquisition, image metadata, automatic background subtraction, external trigger options, and many other useful features. Optional modules for peripheral device control, quantitative imaging, extended third party software interfacing, and extended depth of focus processing can be added to the SPOT 5.0 Advanced software to support specialized applications. |
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MetaMorph Image Analysis SoftwareMetaMorph is designed for demanding quantitative needs in research applications. Its toolbox includes cell counting, deconvolution, image stitching, ratio and calcium imaging, FRET/FRAP, and multi-dimensional imaging. MetaMorph controls SPOT cameras through a custom driver and can also control other manufacturer's cameras and peripherals. |
SPOT Basic
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SPOT Advanced with Modules |
MetaMorph |
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| Full Screen Live Image Preview | √ |
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| Image Annotations Including Lines, Arrows, Circles, Shapes, Pictures, Text | √ |
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| Exportable Measurements Including Area, Perimeter, Length, Angle and Diameter | √ |
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| Time Lapse Imaging | √ |
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| Automatic White Balance | √ |
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| Image Magnifier | √ |
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| Live Image Contrast, Saturation and Color Temperature Adjustments | √ |
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| Reticles | √ |
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| Custom and Preconfigured Color Palettes for Coloring Monochrome Images | √ |
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| Menu-driven Macro Scripting | √ |
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| User-defined Floating Taskbars | √ |
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| Exportable Database | √ |
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| Automatic Background Subtraction | √ |
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| Image Meta Data | √ | √
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| Custom Dialog Box Creation | √ | √
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| Support for Extension Modules | √ | √
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| Saturated Pixel Display | √ | √
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| Peripheral Device Control | √ | √
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| Extended Depth of Focus | √ | √
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| Intensity Display Scaling | √ | √
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| Cell Counting with Thresholding | √
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| Image Stitching | √
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| Deconvolution | √
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| Brightness Measurements | √
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| FISH/FRAP/FRET and Colocalization Studies | √
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| System Requirements | |||||||||||||
PC or Mac |
PC or Mac |
PC only
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| Price | |||||||||||||
included with SPOT camera purchase or $149 for updates |
starts at $499 for SPOT Advanced without modules |
starts at $2300 for offline version without acquisition module
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Key: *: Best Choice for Application √: Capable +: Capability Surpasses Application x: Not Recommended |
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SPOT Basic
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SPOT Advanced with Modules |
MetaMorph |
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Documentation |
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Routine Fluorescence |
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Extreme Low Light Fluorescence |
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Analysis |
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x |
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